YNL194C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 23 7.6%
Arg[R] 18 6.0%
Asn[N] 18 6.0%
Asp[D] 6 2.0%
Cys[C] 6 2.0%
Gln[Q] 9 3.0%
Glu[E] 8 2.7%
Gly[G] 16 5.3%
His[H] 5 1.7%
Ile[I] 15 5.0%
Leu[L] 27 9.0%
Lys[K] 13 4.3%
Met[M] 6 2.0%
Phe[F] 22 7.3%
Pro[P] 13 4.3%
Ser[S] 26 8.6%
Thr[T] 27 9.0%
Trp[W] 7 2.3%
Tyr[Y] 14 4.7%
Val[V] 22 7.3%
Total Length 301

Instability index
Instability index (II) 48.02
This classifies the protein as unstable

Atomic composition
Carbon 1560
Hydrogen 2386
Nitrogen 412
Oxygen 424
Sulfur 12
Total number of atoms 4794
Formula = C1560H2386N412O424S12

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 59735 M-1 cm-1
assuming NO Cys residues appear as half cystines 59360 M-1 cm-1
assuming all Cys residues are reduced 59360 M-1 cm-1
assuming all pairs of Cys residues form cystines 59735 M-1 cm-1

Aliphatic index
Aliphatic index 83.26

Coding Region Translation Calculations
Codon Bias 0.085
Codon Adaptation Index 0.115
Frequency of Optimal Codons 0.448
Hydropathicity of Protein 0.031
Aromaticity Score 0.143