Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
22 |
3.4% |
| Arg[R] |
26 |
4.0% |
| Asn[N] |
82 |
12.7% |
| Asp[D] |
24 |
3.7% |
| Cys[C] |
3 |
0.5% |
| Gln[Q] |
21 |
3.2% |
| Glu[E] |
29 |
4.5% |
| Gly[G] |
28 |
4.3% |
| His[H] |
14 |
2.2% |
| Ile[I] |
34 |
5.3% |
| Leu[L] |
44 |
6.8% |
| Lys[K] |
39 |
6.0% |
| Met[M] |
8 |
1.2% |
| Phe[F] |
16 |
2.5% |
| Pro[P] |
46 |
7.1% |
| Ser[S] |
110 |
17.0% |
| Thr[T] |
57 |
8.8% |
| Trp[W] |
0 |
0.0% |
| Tyr[Y] |
12 |
1.9% |
| Val[V] |
32 |
4.9% |
| Total Length |
647 |
|
| Instability index (II) |
54.82 |
| This classifies the protein as unstable |
|
| Carbon |
2987 |
| Hydrogen |
4813 |
| Nitrogen |
895 |
| Oxygen |
1036 |
| Sulfur |
11 |
| Total number of atoms |
9742 |
| Formula = C2987H4813N895O1036S11 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
18005 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
17880 M-1 cm-1 |
| assuming all Cys residues are reduced |
17880 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
18005 M-1 cm-1 |
|
|
| Codon Bias |
0.004 |
| Codon Adaptation Index |
0.113 |
| Frequency of Optimal Codons |
0.408 |
| Hydropathicity of Protein |
-0.814 |
| Aromaticity Score |
0.043 |
|
