Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
61 |
5.5% |
| Arg[R] |
54 |
4.9% |
| Asn[N] |
48 |
4.3% |
| Asp[D] |
71 |
6.4% |
| Cys[C] |
17 |
1.5% |
| Gln[Q] |
42 |
3.8% |
| Glu[E] |
102 |
9.2% |
| Gly[G] |
61 |
5.5% |
| His[H] |
21 |
1.9% |
| Ile[I] |
76 |
6.8% |
| Leu[L] |
115 |
10.4% |
| Lys[K] |
71 |
6.4% |
| Met[M] |
24 |
2.2% |
| Phe[F] |
42 |
3.8% |
| Pro[P] |
51 |
4.6% |
| Ser[S] |
92 |
8.3% |
| Thr[T] |
52 |
4.7% |
| Trp[W] |
9 |
0.8% |
| Tyr[Y] |
22 |
2.0% |
| Val[V] |
79 |
7.1% |
| Total Length |
1110 |
|
| Instability index (II) |
48.98 |
| This classifies the protein as unstable |
|
| Carbon |
5503 |
| Hydrogen |
8790 |
| Nitrogen |
1484 |
| Oxygen |
1713 |
| Sulfur |
41 |
| Total number of atoms |
17531 |
| Formula = C5503H8790N1484O1713S41 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
83280 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
82280 M-1 cm-1 |
| assuming all Cys residues are reduced |
82280 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
83280 M-1 cm-1 |
|
|
| Codon Bias |
0.079 |
| Codon Adaptation Index |
0.151 |
| Frequency of Optimal Codons |
0.452 |
| Hydropathicity of Protein |
-0.301 |
| Aromaticity Score |
0.066 |
|
