Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
34 |
6.2% |
| Arg[R] |
26 |
4.8% |
| Asn[N] |
39 |
7.1% |
| Asp[D] |
29 |
5.3% |
| Cys[C] |
3 |
0.5% |
| Gln[Q] |
61 |
11.2% |
| Glu[E] |
25 |
4.6% |
| Gly[G] |
40 |
7.3% |
| His[H] |
19 |
3.5% |
| Ile[I] |
21 |
3.8% |
| Leu[L] |
44 |
8.1% |
| Lys[K] |
38 |
7.0% |
| Met[M] |
12 |
2.2% |
| Phe[F] |
16 |
2.9% |
| Pro[P] |
28 |
5.1% |
| Ser[S] |
37 |
6.8% |
| Thr[T] |
23 |
4.2% |
| Trp[W] |
4 |
0.7% |
| Tyr[Y] |
27 |
4.9% |
| Val[V] |
20 |
3.7% |
| Total Length |
546 |
|
| Instability index (II) |
46.05 |
| This classifies the protein as unstable |
|
| Carbon |
2715 |
| Hydrogen |
4222 |
| Nitrogen |
804 |
| Oxygen |
842 |
| Sulfur |
15 |
| Total number of atoms |
8598 |
| Formula = C2715H4222N804O842S15 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
62355 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
62230 M-1 cm-1 |
| assuming all Cys residues are reduced |
62230 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
62355 M-1 cm-1 |
|
|
| Codon Bias |
0.125 |
| Codon Adaptation Index |
0.173 |
| Frequency of Optimal Codons |
0.477 |
| Hydropathicity of Protein |
-0.967 |
| Aromaticity Score |
0.086 |
|
