Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
29 |
3.4% |
| Arg[R] |
37 |
4.4% |
| Asn[N] |
66 |
7.8% |
| Asp[D] |
36 |
4.3% |
| Cys[C] |
4 |
0.5% |
| Gln[Q] |
31 |
3.7% |
| Glu[E] |
57 |
6.7% |
| Gly[G] |
30 |
3.5% |
| His[H] |
18 |
2.1% |
| Ile[I] |
68 |
8.0% |
| Leu[L] |
107 |
12.6% |
| Lys[K] |
55 |
6.5% |
| Met[M] |
18 |
2.1% |
| Phe[F] |
50 |
5.9% |
| Pro[P] |
29 |
3.4% |
| Ser[S] |
78 |
9.2% |
| Thr[T] |
44 |
5.2% |
| Trp[W] |
5 |
0.6% |
| Tyr[Y] |
45 |
5.3% |
| Val[V] |
39 |
4.6% |
| Total Length |
846 |
|
| Instability index (II) |
40.03 |
| This classifies the protein as unstable |
|
| Carbon |
4467 |
| Hydrogen |
6954 |
| Nitrogen |
1150 |
| Oxygen |
1297 |
| Sulfur |
22 |
| Total number of atoms |
13890 |
| Formula = C4467H6954N1150O1297S22 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
94800 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
94550 M-1 cm-1 |
| assuming all Cys residues are reduced |
94550 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
94800 M-1 cm-1 |
|
|
| Codon Bias |
-0.024 |
| Codon Adaptation Index |
0.115 |
| Frequency of Optimal Codons |
0.399 |
| Hydropathicity of Protein |
-0.243 |
| Aromaticity Score |
0.118 |
|
