MET4/YNL103W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 36 5.4%
Arg[R] 22 3.3%
Asn[N] 91 13.5%
Asp[D] 50 7.4%
Cys[C] 0 0.0%
Gln[Q] 25 3.7%
Glu[E] 43 6.4%
Gly[G] 29 4.3%
His[H] 27 4.0%
Ile[I] 35 5.2%
Leu[L] 49 7.3%
Lys[K] 44 6.5%
Met[M] 6 0.9%
Phe[F] 23 3.4%
Pro[P] 30 4.5%
Ser[S] 83 12.4%
Thr[T] 43 6.4%
Trp[W] 1 0.1%
Tyr[Y] 9 1.3%
Val[V] 26 3.9%
Total Length 672

Instability index
Instability index (II) 41.74
This classifies the protein as unstable

Atomic composition
Carbon 3162
Hydrogen 4983
Nitrogen 953
Oxygen 1110
Sulfur 6
Total number of atoms 10214
Formula = C3162H4983N953O1110S6

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 18910 M-1 cm-1

Aliphatic index
Aliphatic index 65.33

Coding Region Translation Calculations
Codon Bias 0.002
Codon Adaptation Index 0.125
Frequency of Optimal Codons 0.418
Hydropathicity of Protein -0.988
Aromaticity Score 0.049