Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
66 |
4.5% |
| Arg[R] |
86 |
5.9% |
| Asn[N] |
94 |
6.4% |
| Asp[D] |
119 |
8.1% |
| Cys[C] |
29 |
2.0% |
| Gln[Q] |
60 |
4.1% |
| Glu[E] |
95 |
6.5% |
| Gly[G] |
68 |
4.6% |
| His[H] |
22 |
1.5% |
| Ile[I] |
87 |
5.9% |
| Leu[L] |
145 |
9.9% |
| Lys[K] |
111 |
7.6% |
| Met[M] |
35 |
2.4% |
| Phe[F] |
46 |
3.1% |
| Pro[P] |
68 |
4.6% |
| Ser[S] |
112 |
7.6% |
| Thr[T] |
78 |
5.3% |
| Trp[W] |
7 |
0.5% |
| Tyr[Y] |
49 |
3.3% |
| Val[V] |
91 |
6.2% |
| Total Length |
1468 |
|
| Instability index (II) |
45.49 |
| This classifies the protein as unstable |
|
| Carbon |
7304 |
| Hydrogen |
11696 |
| Nitrogen |
2042 |
| Oxygen |
2290 |
| Sulfur |
64 |
| Total number of atoms |
23396 |
| Formula = C7304H11696N2042O2290S64 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
113260 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
111510 M-1 cm-1 |
| assuming all Cys residues are reduced |
111510 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
113260 M-1 cm-1 |
|
|
| Codon Bias |
0.061 |
| Codon Adaptation Index |
0.157 |
| Frequency of Optimal Codons |
0.444 |
| Hydropathicity of Protein |
-0.557 |
| Aromaticity Score |
0.069 |
|
