Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
65 |
4.6% |
| Arg[R] |
60 |
4.2% |
| Asn[N] |
80 |
5.6% |
| Asp[D] |
91 |
6.4% |
| Cys[C] |
9 |
0.6% |
| Gln[Q] |
50 |
3.5% |
| Glu[E] |
133 |
9.3% |
| Gly[G] |
78 |
5.5% |
| His[H] |
23 |
1.6% |
| Ile[I] |
116 |
8.1% |
| Leu[L] |
115 |
8.1% |
| Lys[K] |
159 |
11.1% |
| Met[M] |
28 |
2.0% |
| Phe[F] |
52 |
3.6% |
| Pro[P] |
56 |
3.9% |
| Ser[S] |
94 |
6.6% |
| Thr[T] |
83 |
5.8% |
| Trp[W] |
15 |
1.1% |
| Tyr[Y] |
60 |
4.2% |
| Val[V] |
61 |
4.3% |
| Total Length |
1428 |
|
| Instability index (II) |
40.03 |
| This classifies the protein as unstable |
|
| Carbon |
7327 |
| Hydrogen |
11604 |
| Nitrogen |
1958 |
| Oxygen |
2244 |
| Sulfur |
37 |
| Total number of atoms |
23170 |
| Formula = C7327H11604N1958O2244S37 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
172400 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
171900 M-1 cm-1 |
| assuming all Cys residues are reduced |
171900 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
172400 M-1 cm-1 |
|
|
| Codon Bias |
0.084 |
| Codon Adaptation Index |
0.175 |
| Frequency of Optimal Codons |
0.473 |
| Hydropathicity of Protein |
-0.697 |
| Aromaticity Score |
0.089 |
|
