TOM7/YNL070W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 2 3.3%
Arg[R] 2 3.3%
Asn[N] 4 6.7%
Asp[D] 1 1.7%
Cys[C] 0 0.0%
Gln[Q] 0 0.0%
Glu[E] 2 3.3%
Gly[G] 2 3.3%
His[H] 3 5.0%
Ile[I] 4 6.7%
Leu[L] 10 16.7%
Lys[K] 2 3.3%
Met[M] 1 1.7%
Phe[F] 4 6.7%
Pro[P] 5 8.3%
Ser[S] 8 13.3%
Thr[T] 3 5.0%
Trp[W] 2 3.3%
Tyr[Y] 2 3.3%
Val[V] 3 5.0%
Total Length 60

Instability index
Instability index (II) 56.35
This classifies the protein as unstable

Atomic composition
Carbon 323
Hydrogen 490
Nitrogen 80
Oxygen 84
Sulfur 1
Total number of atoms 978
Formula = C323H490N80O84S1

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 13980 M-1 cm-1

Aliphatic index
Aliphatic index 108.83

Coding Region Translation Calculations
Codon Bias 0.209
Codon Adaptation Index 0.223
Frequency of Optimal Codons 0.509
Hydropathicity of Protein 0.212
Aromaticity Score 0.133