Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
63 |
5.4% |
| Arg[R] |
36 |
3.1% |
| Asn[N] |
161 |
13.8% |
| Asp[D] |
85 |
7.3% |
| Cys[C] |
4 |
0.3% |
| Gln[Q] |
43 |
3.7% |
| Glu[E] |
54 |
4.6% |
| Gly[G] |
44 |
3.8% |
| His[H] |
38 |
3.3% |
| Ile[I] |
55 |
4.7% |
| Leu[L] |
84 |
7.2% |
| Lys[K] |
70 |
6.0% |
| Met[M] |
21 |
1.8% |
| Phe[F] |
30 |
2.6% |
| Pro[P] |
63 |
5.4% |
| Ser[S] |
154 |
13.2% |
| Thr[T] |
77 |
6.6% |
| Trp[W] |
2 |
0.2% |
| Tyr[Y] |
26 |
2.2% |
| Val[V] |
55 |
4.7% |
| Total Length |
1165 |
|
| Instability index (II) |
46.09 |
| This classifies the protein as unstable |
|
| Carbon |
5447 |
| Hydrogen |
8607 |
| Nitrogen |
1625 |
| Oxygen |
1905 |
| Sulfur |
25 |
| Total number of atoms |
17609 |
| Formula = C5447H8607N1625O1905S25 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
49990 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
49740 M-1 cm-1 |
| assuming all Cys residues are reduced |
49740 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
49990 M-1 cm-1 |
|
|
| Codon Bias |
-0.016 |
| Codon Adaptation Index |
0.124 |
| Frequency of Optimal Codons |
0.404 |
| Hydropathicity of Protein |
-0.895 |
| Aromaticity Score |
0.050 |
|
