Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
19 |
3.9% |
| Arg[R] |
20 |
4.1% |
| Asn[N] |
24 |
4.9% |
| Asp[D] |
25 |
5.1% |
| Cys[C] |
4 |
0.8% |
| Gln[Q] |
13 |
2.7% |
| Glu[E] |
29 |
5.9% |
| Gly[G] |
17 |
3.5% |
| His[H] |
14 |
2.9% |
| Ile[I] |
27 |
5.5% |
| Leu[L] |
49 |
10.0% |
| Lys[K] |
32 |
6.5% |
| Met[M] |
15 |
3.1% |
| Phe[F] |
14 |
2.9% |
| Pro[P] |
39 |
8.0% |
| Ser[S] |
72 |
14.7% |
| Thr[T] |
35 |
7.2% |
| Trp[W] |
2 |
0.4% |
| Tyr[Y] |
13 |
2.7% |
| Val[V] |
26 |
5.3% |
| Total Length |
489 |
|
| Instability index (II) |
46.54 |
| This classifies the protein as unstable |
|
| Carbon |
2382 |
| Hydrogen |
3812 |
| Nitrogen |
648 |
| Oxygen |
755 |
| Sulfur |
19 |
| Total number of atoms |
7616 |
| Formula = C2382H3812N648O755S19 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
30620 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
30370 M-1 cm-1 |
| assuming all Cys residues are reduced |
30370 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
30620 M-1 cm-1 |
|
|
| Codon Bias |
0.022 |
| Codon Adaptation Index |
0.103 |
| Frequency of Optimal Codons |
0.409 |
| Hydropathicity of Protein |
-0.448 |
| Aromaticity Score |
0.059 |
|
