Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
18 |
5.6% |
| Arg[R] |
14 |
4.3% |
| Asn[N] |
11 |
3.4% |
| Asp[D] |
14 |
4.3% |
| Cys[C] |
7 |
2.2% |
| Gln[Q] |
19 |
5.9% |
| Glu[E] |
19 |
5.9% |
| Gly[G] |
6 |
1.9% |
| His[H] |
13 |
4.0% |
| Ile[I] |
29 |
9.0% |
| Leu[L] |
39 |
12.1% |
| Lys[K] |
17 |
5.3% |
| Met[M] |
6 |
1.9% |
| Phe[F] |
12 |
3.7% |
| Pro[P] |
16 |
5.0% |
| Ser[S] |
25 |
7.7% |
| Thr[T] |
18 |
5.6% |
| Trp[W] |
7 |
2.2% |
| Tyr[Y] |
18 |
5.6% |
| Val[V] |
15 |
4.6% |
| Total Length |
323 |
|
| Instability index (II) |
57.93 |
| This classifies the protein as unstable |
|
| Carbon |
1728 |
| Hydrogen |
2669 |
| Nitrogen |
445 |
| Oxygen |
481 |
| Sulfur |
13 |
| Total number of atoms |
5336 |
| Formula = C1728H2669N445O481S13 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
65695 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
65320 M-1 cm-1 |
| assuming all Cys residues are reduced |
65320 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
65695 M-1 cm-1 |
|
|
| Codon Bias |
0.055 |
| Codon Adaptation Index |
0.139 |
| Frequency of Optimal Codons |
0.445 |
| Hydropathicity of Protein |
-0.140 |
| Aromaticity Score |
0.115 |
|
