PBI2/YNL015W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 2 2.7%
Arg[R] 0 0.0%
Asn[N] 6 8.0%
Asp[D] 6 8.0%
Cys[C] 0 0.0%
Gln[Q] 0 0.0%
Glu[E] 7 9.3%
Gly[G] 3 4.0%
His[H] 6 8.0%
Ile[I] 6 8.0%
Leu[L] 5 6.7%
Lys[K] 11 14.7%
Met[M] 1 1.3%
Phe[F] 3 4.0%
Pro[P] 2 2.7%
Ser[S] 2 2.7%
Thr[T] 5 6.7%
Trp[W] 0 0.0%
Tyr[Y] 1 1.3%
Val[V] 9 12.0%
Total Length 75

Instability index
Instability index (II) 14.13
This classifies the protein as stable

Atomic composition
Carbon 385
Hydrogen 616
Nitrogen 104
Oxygen 116
Sulfur 1
Total number of atoms 1222
Formula = C385H616N104O116S1

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 1490 M-1 cm-1

Aliphatic index
Aliphatic index 94.67

Coding Region Translation Calculations
Codon Bias 0.421
Codon Adaptation Index 0.371
Frequency of Optimal Codons 0.689
Hydropathicity of Protein -0.556
Aromaticity Score 0.053