SIS1/YNL007C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 18 5.1%
Arg[R] 12 3.4%
Asn[N] 12 3.4%
Asp[D] 18 5.1%
Cys[C] 0 0.0%
Gln[Q] 18 5.1%
Glu[E] 18 5.1%
Gly[G] 56 15.9%
His[H] 5 1.4%
Ile[I] 15 4.3%
Leu[L] 21 6.0%
Lys[K] 29 8.2%
Met[M] 8 2.3%
Phe[F] 21 6.0%
Pro[P] 28 8.0%
Ser[S] 31 8.8%
Thr[T] 17 4.8%
Trp[W] 1 0.3%
Tyr[Y] 13 3.7%
Val[V] 11 3.1%
Total Length 352

Instability index
Instability index (II) 47.40
This classifies the protein as unstable

Atomic composition
Carbon 1671
Hydrogen 2572
Nitrogen 458
Oxygen 516
Sulfur 8
Total number of atoms 5225
Formula = C1671H2572N458O516S8

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 24870 M-1 cm-1

Aliphatic index
Aliphatic index 54.06

Coding Region Translation Calculations
Codon Bias 0.155
Codon Adaptation Index 0.161
Frequency of Optimal Codons 0.481
Hydropathicity of Protein -0.670
Aromaticity Score 0.099