Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
31 |
5.6% |
| Arg[R] |
30 |
5.4% |
| Asn[N] |
24 |
4.3% |
| Asp[D] |
32 |
5.8% |
| Cys[C] |
9 |
1.6% |
| Gln[Q] |
17 |
3.1% |
| Glu[E] |
35 |
6.3% |
| Gly[G] |
38 |
6.9% |
| His[H] |
10 |
1.8% |
| Ile[I] |
53 |
9.6% |
| Leu[L] |
41 |
7.4% |
| Lys[K] |
23 |
4.2% |
| Met[M] |
13 |
2.4% |
| Phe[F] |
28 |
5.1% |
| Pro[P] |
19 |
3.4% |
| Ser[S] |
34 |
6.2% |
| Thr[T] |
36 |
6.5% |
| Trp[W] |
16 |
2.9% |
| Tyr[Y] |
19 |
3.4% |
| Val[V] |
44 |
8.0% |
| Total Length |
552 |
|
| Instability index (II) |
35.42 |
| This classifies the protein as stable |
|
| Carbon |
2847 |
| Hydrogen |
4392 |
| Nitrogen |
742 |
| Oxygen |
817 |
| Sulfur |
22 |
| Total number of atoms |
8820 |
| Formula = C2847H4392N742O817S22 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
116810 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
116310 M-1 cm-1 |
| assuming all Cys residues are reduced |
116310 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
116810 M-1 cm-1 |
|
|
| Codon Bias |
0.207 |
| Codon Adaptation Index |
0.198 |
| Frequency of Optimal Codons |
0.539 |
| Hydropathicity of Protein |
-0.020 |
| Aromaticity Score |
0.114 |
|
