Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
26 |
4.0% |
| Arg[R] |
30 |
4.7% |
| Asn[N] |
43 |
6.7% |
| Asp[D] |
24 |
3.7% |
| Cys[C] |
12 |
1.9% |
| Gln[Q] |
22 |
3.4% |
| Glu[E] |
41 |
6.4% |
| Gly[G] |
27 |
4.2% |
| His[H] |
12 |
1.9% |
| Ile[I] |
54 |
8.4% |
| Leu[L] |
80 |
12.5% |
| Lys[K] |
43 |
6.7% |
| Met[M] |
14 |
2.2% |
| Phe[F] |
27 |
4.2% |
| Pro[P] |
32 |
5.0% |
| Ser[S] |
43 |
6.7% |
| Thr[T] |
39 |
6.1% |
| Trp[W] |
7 |
1.1% |
| Tyr[Y] |
27 |
4.2% |
| Val[V] |
39 |
6.1% |
| Total Length |
642 |
|
| Instability index (II) |
48.20 |
| This classifies the protein as unstable |
|
| Carbon |
3338 |
| Hydrogen |
5293 |
| Nitrogen |
871 |
| Oxygen |
947 |
| Sulfur |
26 |
| Total number of atoms |
10475 |
| Formula = C3338H5293N871O947S26 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
79480 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
78730 M-1 cm-1 |
| assuming all Cys residues are reduced |
78730 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
79480 M-1 cm-1 |
|
|
| Codon Bias |
0.029 |
| Codon Adaptation Index |
0.119 |
| Frequency of Optimal Codons |
0.427 |
| Hydropathicity of Protein |
-0.111 |
| Aromaticity Score |
0.095 |
|
