Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
103 |
9.5% |
| Arg[R] |
32 |
2.9% |
| Asn[N] |
52 |
4.8% |
| Asp[D] |
71 |
6.5% |
| Cys[C] |
15 |
1.4% |
| Gln[Q] |
50 |
4.6% |
| Glu[E] |
77 |
7.1% |
| Gly[G] |
38 |
3.5% |
| His[H] |
16 |
1.5% |
| Ile[I] |
84 |
7.7% |
| Leu[L] |
132 |
12.1% |
| Lys[K] |
54 |
5.0% |
| Met[M] |
25 |
2.3% |
| Phe[F] |
43 |
3.9% |
| Pro[P] |
46 |
4.2% |
| Ser[S] |
82 |
7.5% |
| Thr[T] |
60 |
5.5% |
| Trp[W] |
11 |
1.0% |
| Tyr[Y] |
30 |
2.8% |
| Val[V] |
68 |
6.2% |
| Total Length |
1089 |
|
| Instability index (II) |
45.34 |
| This classifies the protein as unstable |
|
| Carbon |
5424 |
| Hydrogen |
8588 |
| Nitrogen |
1384 |
| Oxygen |
1660 |
| Sulfur |
40 |
| Total number of atoms |
17096 |
| Formula = C5424H8588N1384O1660S40 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
106075 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
105200 M-1 cm-1 |
| assuming all Cys residues are reduced |
105200 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
106075 M-1 cm-1 |
|
|
| Codon Bias |
0.151 |
| Codon Adaptation Index |
0.180 |
| Frequency of Optimal Codons |
0.494 |
| Hydropathicity of Protein |
0.027 |
| Aromaticity Score |
0.077 |
|
