Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
92 |
5.2% |
| Arg[R] |
82 |
4.6% |
| Asn[N] |
80 |
4.5% |
| Asp[D] |
70 |
3.9% |
| Cys[C] |
26 |
1.5% |
| Gln[Q] |
61 |
3.4% |
| Glu[E] |
103 |
5.8% |
| Gly[G] |
89 |
5.0% |
| His[H] |
38 |
2.1% |
| Ile[I] |
132 |
7.4% |
| Leu[L] |
206 |
11.5% |
| Lys[K] |
100 |
5.6% |
| Met[M] |
43 |
2.4% |
| Phe[F] |
154 |
8.6% |
| Pro[P] |
75 |
4.2% |
| Ser[S] |
133 |
7.5% |
| Thr[T] |
96 |
5.4% |
| Trp[W] |
39 |
2.2% |
| Tyr[Y] |
81 |
4.5% |
| Val[V] |
85 |
4.8% |
| Total Length |
1785 |
|
| Instability index (II) |
35.56 |
| This classifies the protein as stable |
|
| Carbon |
9642 |
| Hydrogen |
14585 |
| Nitrogen |
2387 |
| Oxygen |
2583 |
| Sulfur |
69 |
| Total number of atoms |
29266 |
| Formula = C9642H14585N2387O2583S69 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
336815 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
335190 M-1 cm-1 |
| assuming all Cys residues are reduced |
335190 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
336815 M-1 cm-1 |
|
|
| Codon Bias |
0.038 |
| Codon Adaptation Index |
0.126 |
| Frequency of Optimal Codons |
0.429 |
| Hydropathicity of Protein |
0.016 |
| Aromaticity Score |
0.154 |
|
