PRC1/YMR297W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 31 5.8%
Arg[R] 11 2.1%
Asn[N] 34 6.4%
Asp[D] 43 8.1%
Cys[C] 12 2.3%
Gln[Q] 17 3.2%
Glu[E] 32 6.0%
Gly[G] 38 7.1%
His[H] 11 2.1%
Ile[I] 24 4.5%
Leu[L] 54 10.2%
Lys[K] 29 5.5%
Met[M] 7 1.3%
Phe[F] 28 5.3%
Pro[P] 28 5.3%
Ser[S] 36 6.8%
Thr[T] 24 4.5%
Trp[W] 12 2.3%
Tyr[Y] 26 4.9%
Val[V] 35 6.6%
Total Length 532

Instability index
Instability index (II) 39.49
This classifies the protein as stable

Atomic composition
Carbon 2704
Hydrogen 4053
Nitrogen 679
Oxygen 820
Sulfur 19
Total number of atoms 8275
Formula = C2704H4053N679O820S19

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 105490 M-1 cm-1
assuming NO Cys residues appear as half cystines 104740 M-1 cm-1
assuming all Cys residues are reduced 104740 M-1 cm-1
assuming all pairs of Cys residues form cystines 105490 M-1 cm-1

Aliphatic index
Aliphatic index 82.09

Coding Region Translation Calculations
Codon Bias 0.343
Codon Adaptation Index 0.257
Frequency of Optimal Codons 0.610
Hydropathicity of Protein -0.284
Aromaticity Score 0.124