Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
12 |
3.2% |
| Arg[R] |
25 |
6.7% |
| Asn[N] |
21 |
5.6% |
| Asp[D] |
29 |
7.8% |
| Cys[C] |
2 |
0.5% |
| Gln[Q] |
27 |
7.2% |
| Glu[E] |
52 |
13.9% |
| Gly[G] |
11 |
2.9% |
| His[H] |
2 |
0.5% |
| Ile[I] |
27 |
7.2% |
| Leu[L] |
44 |
11.8% |
| Lys[K] |
35 |
9.4% |
| Met[M] |
9 |
2.4% |
| Phe[F] |
7 |
1.9% |
| Pro[P] |
6 |
1.6% |
| Ser[S] |
28 |
7.5% |
| Thr[T] |
12 |
3.2% |
| Trp[W] |
1 |
0.3% |
| Tyr[Y] |
6 |
1.6% |
| Val[V] |
17 |
4.6% |
| Total Length |
373 |
|
| Instability index (II) |
54.10 |
| This classifies the protein as unstable |
|
| Carbon |
1877 |
| Hydrogen |
3098 |
| Nitrogen |
536 |
| Oxygen |
630 |
| Sulfur |
11 |
| Total number of atoms |
6152 |
| Formula = C1877H3098N536O630S11 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
14565 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
14440 M-1 cm-1 |
| assuming all Cys residues are reduced |
14440 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
14565 M-1 cm-1 |
|
|
| Codon Bias |
0.085 |
| Codon Adaptation Index |
0.145 |
| Frequency of Optimal Codons |
0.466 |
| Hydropathicity of Protein |
-0.903 |
| Aromaticity Score |
0.038 |
|
