Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
27 |
4.7% |
| Arg[R] |
40 |
6.9% |
| Asn[N] |
27 |
4.7% |
| Asp[D] |
38 |
6.6% |
| Cys[C] |
11 |
1.9% |
| Gln[Q] |
19 |
3.3% |
| Glu[E] |
26 |
4.5% |
| Gly[G] |
22 |
3.8% |
| His[H] |
15 |
2.6% |
| Ile[I] |
48 |
8.3% |
| Leu[L] |
62 |
10.7% |
| Lys[K] |
54 |
9.3% |
| Met[M] |
12 |
2.1% |
| Phe[F] |
31 |
5.3% |
| Pro[P] |
18 |
3.1% |
| Ser[S] |
50 |
8.6% |
| Thr[T] |
22 |
3.8% |
| Trp[W] |
9 |
1.6% |
| Tyr[Y] |
20 |
3.4% |
| Val[V] |
29 |
5.0% |
| Total Length |
580 |
|
| Instability index (II) |
42.68 |
| This classifies the protein as unstable |
|
| Carbon |
3048 |
| Hydrogen |
4835 |
| Nitrogen |
839 |
| Oxygen |
847 |
| Sulfur |
23 |
| Total number of atoms |
9592 |
| Formula = C3048H4835N839O847S23 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
79925 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
79300 M-1 cm-1 |
| assuming all Cys residues are reduced |
79300 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
79925 M-1 cm-1 |
|
|
| Codon Bias |
-0.019 |
| Codon Adaptation Index |
0.116 |
| Frequency of Optimal Codons |
0.397 |
| Hydropathicity of Protein |
-0.330 |
| Aromaticity Score |
0.103 |
|
