CAT8/YMR280C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 75 5.2%
Arg[R] 63 4.4%
Asn[N] 143 10.0%
Asp[D] 83 5.8%
Cys[C] 16 1.1%
Gln[Q] 63 4.4%
Glu[E] 60 4.2%
Gly[G] 59 4.1%
His[H] 26 1.8%
Ile[I] 71 5.0%
Leu[L] 159 11.1%
Lys[K] 107 7.5%
Met[M] 37 2.6%
Phe[F] 60 4.2%
Pro[P] 70 4.9%
Ser[S] 150 10.5%
Thr[T] 98 6.8%
Trp[W] 5 0.3%
Tyr[Y] 34 2.4%
Val[V] 54 3.8%
Total Length 1433

Instability index
Instability index (II) 38.27
This classifies the protein as stable

Atomic composition
Carbon 7014
Hydrogen 11224
Nitrogen 1992
Oxygen 2208
Sulfur 53
Total number of atoms 22491
Formula = C7014H11224N1992O2208S53

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 79160 M-1 cm-1
assuming NO Cys residues appear as half cystines 78160 M-1 cm-1
assuming all Cys residues are reduced 78160 M-1 cm-1
assuming all pairs of Cys residues form cystines 79160 M-1 cm-1

Aliphatic index
Aliphatic index 78.76

Coding Region Translation Calculations
Codon Bias 0.031
Codon Adaptation Index 0.132
Frequency of Optimal Codons 0.419
Hydropathicity of Protein -0.568
Aromaticity Score 0.069