Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
28 |
3.8% |
| Arg[R] |
25 |
3.4% |
| Asn[N] |
36 |
4.9% |
| Asp[D] |
77 |
10.5% |
| Cys[C] |
9 |
1.2% |
| Gln[Q] |
42 |
5.7% |
| Glu[E] |
60 |
8.2% |
| Gly[G] |
36 |
4.9% |
| His[H] |
16 |
2.2% |
| Ile[I] |
41 |
5.6% |
| Leu[L] |
70 |
9.6% |
| Lys[K] |
51 |
7.0% |
| Met[M] |
15 |
2.0% |
| Phe[F] |
22 |
3.0% |
| Pro[P] |
40 |
5.5% |
| Ser[S] |
46 |
6.3% |
| Thr[T] |
42 |
5.7% |
| Trp[W] |
10 |
1.4% |
| Tyr[Y] |
22 |
3.0% |
| Val[V] |
44 |
6.0% |
| Total Length |
732 |
|
| Instability index (II) |
42.74 |
| This classifies the protein as unstable |
|
| Carbon |
3670 |
| Hydrogen |
5728 |
| Nitrogen |
978 |
| Oxygen |
1195 |
| Sulfur |
24 |
| Total number of atoms |
11595 |
| Formula = C3670H5728N978O1195S24 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
88280 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
87780 M-1 cm-1 |
| assuming all Cys residues are reduced |
87780 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
88280 M-1 cm-1 |
|
|
| Codon Bias |
0.075 |
| Codon Adaptation Index |
0.149 |
| Frequency of Optimal Codons |
0.461 |
| Hydropathicity of Protein |
-0.682 |
| Aromaticity Score |
0.074 |
|
