Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
60 |
3.8% |
| Arg[R] |
51 |
3.3% |
| Asn[N] |
114 |
7.3% |
| Asp[D] |
97 |
6.2% |
| Cys[C] |
21 |
1.3% |
| Gln[Q] |
60 |
3.8% |
| Glu[E] |
104 |
6.7% |
| Gly[G] |
51 |
3.3% |
| His[H] |
19 |
1.2% |
| Ile[I] |
109 |
7.0% |
| Leu[L] |
205 |
13.1% |
| Lys[K] |
118 |
7.6% |
| Met[M] |
28 |
1.8% |
| Phe[F] |
98 |
6.3% |
| Pro[P] |
38 |
2.4% |
| Ser[S] |
142 |
9.1% |
| Thr[T] |
81 |
5.2% |
| Trp[W] |
17 |
1.1% |
| Tyr[Y] |
63 |
4.0% |
| Val[V] |
86 |
5.5% |
| Total Length |
1562 |
|
| Instability index (II) |
37.31 |
| This classifies the protein as stable |
|
| Carbon |
8167 |
| Hydrogen |
12740 |
| Nitrogen |
2062 |
| Oxygen |
2425 |
| Sulfur |
49 |
| Total number of atoms |
25443 |
| Formula = C8167H12740N2062O2425S49 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
188620 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
187370 M-1 cm-1 |
| assuming all Cys residues are reduced |
187370 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
188620 M-1 cm-1 |
|
|
| Codon Bias |
0.028 |
| Codon Adaptation Index |
0.146 |
| Frequency of Optimal Codons |
0.432 |
| Hydropathicity of Protein |
-0.188 |
| Aromaticity Score |
0.114 |
|