RKR1/YMR247C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 60 3.8%
Arg[R] 51 3.3%
Asn[N] 114 7.3%
Asp[D] 97 6.2%
Cys[C] 21 1.3%
Gln[Q] 60 3.8%
Glu[E] 104 6.7%
Gly[G] 51 3.3%
His[H] 19 1.2%
Ile[I] 109 7.0%
Leu[L] 205 13.1%
Lys[K] 118 7.6%
Met[M] 28 1.8%
Phe[F] 98 6.3%
Pro[P] 38 2.4%
Ser[S] 142 9.1%
Thr[T] 81 5.2%
Trp[W] 17 1.1%
Tyr[Y] 63 4.0%
Val[V] 86 5.5%
Total Length 1562

Instability index
Instability index (II) 37.31
This classifies the protein as stable

Atomic composition
Carbon 8167
Hydrogen 12740
Nitrogen 2062
Oxygen 2425
Sulfur 49
Total number of atoms 25443
Formula = C8167H12740N2062O2425S49

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 188620 M-1 cm-1
assuming NO Cys residues appear as half cystines 187370 M-1 cm-1
assuming all Cys residues are reduced 187370 M-1 cm-1
assuming all pairs of Cys residues form cystines 188620 M-1 cm-1

Aliphatic index
Aliphatic index 98.21

Coding Region Translation Calculations
Codon Bias 0.028
Codon Adaptation Index 0.146
Frequency of Optimal Codons 0.432
Hydropathicity of Protein -0.188
Aromaticity Score 0.114