RPL20A/YMR242C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 12 7.0%
Arg[R] 16 9.3%
Asn[N] 5 2.9%
Asp[D] 5 2.9%
Cys[C] 0 0.0%
Gln[Q] 7 4.1%
Glu[E] 10 5.8%
Gly[G] 4 2.3%
His[H] 7 4.1%
Ile[I] 10 5.8%
Leu[L] 9 5.2%
Lys[K] 19 11.0%
Met[M] 4 2.3%
Phe[F] 10 5.8%
Pro[P] 7 4.1%
Ser[S] 12 7.0%
Thr[T] 9 5.2%
Trp[W] 2 1.2%
Tyr[Y] 9 5.2%
Val[V] 15 8.7%
Total Length 172

Instability index
Instability index (II) 51.23
This classifies the protein as unstable

Atomic composition
Carbon 930
Hydrogen 1467
Nitrogen 267
Oxygen 245
Sulfur 4
Total number of atoms 2913
Formula = C930H1467N267O245S4

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 24410 M-1 cm-1

Aliphatic index
Aliphatic index 75.35

Coding Region Translation Calculations
Codon Bias 0.774
Codon Adaptation Index 0.721
Frequency of Optimal Codons 0.867
Hydropathicity of Protein -0.615
Aromaticity Score 0.122