YMR221C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 28 5.6%
Arg[R] 18 3.6%
Asn[N] 20 4.0%
Asp[D] 20 4.0%
Cys[C] 11 2.2%
Gln[Q] 15 3.0%
Glu[E] 16 3.2%
Gly[G] 33 6.5%
His[H] 7 1.4%
Ile[I] 42 8.3%
Leu[L] 74 14.7%
Lys[K] 17 3.4%
Met[M] 9 1.8%
Phe[F] 35 6.9%
Pro[P] 23 4.6%
Ser[S] 40 7.9%
Thr[T] 36 7.1%
Trp[W] 6 1.2%
Tyr[Y] 21 4.2%
Val[V] 33 6.5%
Total Length 504

Instability index
Instability index (II) 39.58
This classifies the protein as stable

Atomic composition
Carbon 2605
Hydrogen 4048
Nitrogen 630
Oxygen 709
Sulfur 20
Total number of atoms 8012
Formula = C2605H4048N630O709S20

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 64915 M-1 cm-1
assuming NO Cys residues appear as half cystines 64290 M-1 cm-1
assuming all Cys residues are reduced 64290 M-1 cm-1
assuming all pairs of Cys residues form cystines 64915 M-1 cm-1

Aliphatic index
Aliphatic index 114.31

Coding Region Translation Calculations
Codon Bias 0.138
Codon Adaptation Index 0.146
Frequency of Optimal Codons 0.479
Hydropathicity of Protein 0.484
Aromaticity Score 0.123