Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
35 |
6.7% |
| Arg[R] |
11 |
2.1% |
| Asn[N] |
44 |
8.4% |
| Asp[D] |
39 |
7.4% |
| Cys[C] |
6 |
1.1% |
| Gln[Q] |
12 |
2.3% |
| Glu[E] |
20 |
3.8% |
| Gly[G] |
35 |
6.7% |
| His[H] |
3 |
0.6% |
| Ile[I] |
30 |
5.7% |
| Leu[L] |
42 |
8.0% |
| Lys[K] |
28 |
5.3% |
| Met[M] |
5 |
1.0% |
| Phe[F] |
23 |
4.4% |
| Pro[P] |
18 |
3.4% |
| Ser[S] |
77 |
14.7% |
| Thr[T] |
36 |
6.9% |
| Trp[W] |
2 |
0.4% |
| Tyr[Y] |
30 |
5.7% |
| Val[V] |
28 |
5.3% |
| Total Length |
524 |
|
| Instability index (II) |
36.76 |
| This classifies the protein as stable |
|
| Carbon |
2498 |
| Hydrogen |
3845 |
| Nitrogen |
649 |
| Oxygen |
842 |
| Sulfur |
11 |
| Total number of atoms |
7845 |
| Formula = C2498H3845N649O842S11 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
56075 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
55700 M-1 cm-1 |
| assuming all Cys residues are reduced |
55700 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
56075 M-1 cm-1 |
|
|
| Codon Bias |
0.216 |
| Codon Adaptation Index |
0.217 |
| Frequency of Optimal Codons |
0.536 |
| Hydropathicity of Protein |
-0.338 |
| Aromaticity Score |
0.105 |
|
