Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
109 |
5.1% |
| Arg[R] |
119 |
5.6% |
| Asn[N] |
117 |
5.5% |
| Asp[D] |
118 |
5.6% |
| Cys[C] |
21 |
1.0% |
| Gln[Q] |
74 |
3.5% |
| Glu[E] |
149 |
7.0% |
| Gly[G] |
125 |
5.9% |
| His[H] |
43 |
2.0% |
| Ile[I] |
178 |
8.4% |
| Leu[L] |
209 |
9.8% |
| Lys[K] |
155 |
7.3% |
| Met[M] |
44 |
2.1% |
| Phe[F] |
85 |
4.0% |
| Pro[P] |
92 |
4.3% |
| Ser[S] |
159 |
7.5% |
| Thr[T] |
94 |
4.4% |
| Trp[W] |
25 |
1.2% |
| Tyr[Y] |
81 |
3.8% |
| Val[V] |
126 |
5.9% |
| Total Length |
2123 |
|
| Instability index (II) |
43.45 |
| This classifies the protein as unstable |
|
| Carbon |
10851 |
| Hydrogen |
17171 |
| Nitrogen |
2937 |
| Oxygen |
3183 |
| Sulfur |
65 |
| Total number of atoms |
34207 |
| Formula = C10851H17171N2937O3183S65 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
259440 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
258190 M-1 cm-1 |
| assuming all Cys residues are reduced |
258190 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
259440 M-1 cm-1 |
|
|
| Codon Bias |
0.033 |
| Codon Adaptation Index |
0.122 |
| Frequency of Optimal Codons |
0.428 |
| Hydropathicity of Protein |
-0.332 |
| Aromaticity Score |
0.090 |
|