HFA1/YMR207C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 109 5.1%
Arg[R] 119 5.6%
Asn[N] 117 5.5%
Asp[D] 118 5.6%
Cys[C] 21 1.0%
Gln[Q] 74 3.5%
Glu[E] 149 7.0%
Gly[G] 125 5.9%
His[H] 43 2.0%
Ile[I] 178 8.4%
Leu[L] 209 9.8%
Lys[K] 155 7.3%
Met[M] 44 2.1%
Phe[F] 85 4.0%
Pro[P] 92 4.3%
Ser[S] 159 7.5%
Thr[T] 94 4.4%
Trp[W] 25 1.2%
Tyr[Y] 81 3.8%
Val[V] 126 5.9%
Total Length 2123

Instability index
Instability index (II) 43.45
This classifies the protein as unstable

Atomic composition
Carbon 10851
Hydrogen 17171
Nitrogen 2937
Oxygen 3183
Sulfur 65
Total number of atoms 34207
Formula = C10851H17171N2937O3183S65

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 259440 M-1 cm-1
assuming NO Cys residues appear as half cystines 258190 M-1 cm-1
assuming all Cys residues are reduced 258190 M-1 cm-1
assuming all pairs of Cys residues form cystines 259440 M-1 cm-1

Aliphatic index
Aliphatic index 93.44

Coding Region Translation Calculations
Codon Bias 0.033
Codon Adaptation Index 0.122
Frequency of Optimal Codons 0.428
Hydropathicity of Protein -0.332
Aromaticity Score 0.090