Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
71 |
4.9% |
| Arg[R] |
82 |
5.7% |
| Asn[N] |
104 |
7.2% |
| Asp[D] |
107 |
7.4% |
| Cys[C] |
18 |
1.2% |
| Gln[Q] |
67 |
4.6% |
| Glu[E] |
101 |
7.0% |
| Gly[G] |
62 |
4.3% |
| His[H] |
33 |
2.3% |
| Ile[I] |
91 |
6.3% |
| Leu[L] |
103 |
7.1% |
| Lys[K] |
104 |
7.2% |
| Met[M] |
38 |
2.6% |
| Phe[F] |
50 |
3.5% |
| Pro[P] |
61 |
4.2% |
| Ser[S] |
147 |
10.2% |
| Thr[T] |
93 |
6.4% |
| Trp[W] |
6 |
0.4% |
| Tyr[Y] |
40 |
2.8% |
| Val[V] |
69 |
4.8% |
| Total Length |
1447 |
|
| Instability index (II) |
45.90 |
| This classifies the protein as unstable |
|
| Carbon |
7082 |
| Hydrogen |
11278 |
| Nitrogen |
2040 |
| Oxygen |
2315 |
| Sulfur |
56 |
| Total number of atoms |
22771 |
| Formula = C7082H11278N2040O2315S56 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
93725 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
92600 M-1 cm-1 |
| assuming all Cys residues are reduced |
92600 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
93725 M-1 cm-1 |
|
|
| Codon Bias |
0.027 |
| Codon Adaptation Index |
0.139 |
| Frequency of Optimal Codons |
0.431 |
| Hydropathicity of Protein |
-0.756 |
| Aromaticity Score |
0.066 |
|
