HSC82/YMR186W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 44 6.2%
Arg[R] 27 3.8%
Asn[N] 28 4.0%
Asp[D] 46 6.5%
Cys[C] 0 0.0%
Gln[Q] 22 3.1%
Glu[E] 96 13.6%
Gly[G] 25 3.5%
His[H] 4 0.6%
Ile[I] 47 6.7%
Leu[L] 68 9.6%
Lys[K] 73 10.4%
Met[M] 13 1.8%
Phe[F] 34 4.8%
Pro[P] 27 3.8%
Ser[S] 45 6.4%
Thr[T] 40 5.7%
Trp[W] 5 0.7%
Tyr[Y] 19 2.7%
Val[V] 42 6.0%
Total Length 705

Instability index
Instability index (II) 42.83
This classifies the protein as unstable

Atomic composition
Carbon 3619
Hydrogen 5752
Nitrogen 922
Oxygen 1144
Sulfur 13
Total number of atoms 11450
Formula = C3619H5752N922O1144S13

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 55810 M-1 cm-1

Aliphatic index
Aliphatic index 87.13

Coding Region Translation Calculations
Codon Bias 0.636
Codon Adaptation Index 0.581
Frequency of Optimal Codons 0.789
Hydropathicity of Protein -0.556
Aromaticity Score 0.082