Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
39 |
5.1% |
| Arg[R] |
20 |
2.6% |
| Asn[N] |
108 |
14.2% |
| Asp[D] |
33 |
4.4% |
| Cys[C] |
1 |
0.1% |
| Gln[Q] |
113 |
14.9% |
| Glu[E] |
18 |
2.4% |
| Gly[G] |
29 |
3.8% |
| His[H] |
18 |
2.4% |
| Ile[I] |
29 |
3.8% |
| Leu[L] |
40 |
5.3% |
| Lys[K] |
31 |
4.1% |
| Met[M] |
28 |
3.7% |
| Phe[F] |
32 |
4.2% |
| Pro[P] |
53 |
7.0% |
| Ser[S] |
80 |
10.6% |
| Thr[T] |
45 |
5.9% |
| Trp[W] |
3 |
0.4% |
| Tyr[Y] |
13 |
1.7% |
| Val[V] |
25 |
3.3% |
| Total Length |
758 |
|
| Instability index (II) |
62.38 |
| This classifies the protein as unstable |
|
| Carbon |
3613 |
| Hydrogen |
5627 |
| Nitrogen |
1109 |
| Oxygen |
1220 |
| Sulfur |
29 |
| Total number of atoms |
11598 |
| Formula = C3613H5627N1109O1220S29 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
35870 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
35870 M-1 cm-1 |
| assuming all Cys residues are reduced |
35870 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
35870 M-1 cm-1 |
|
|
| Codon Bias |
0.034 |
| Codon Adaptation Index |
0.133 |
| Frequency of Optimal Codons |
0.439 |
| Hydropathicity of Protein |
-1.094 |
| Aromaticity Score |
0.063 |
|
