Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
21 |
3.0% |
| Arg[R] |
40 |
5.7% |
| Asn[N] |
44 |
6.2% |
| Asp[D] |
39 |
5.5% |
| Cys[C] |
13 |
1.8% |
| Gln[Q] |
25 |
3.5% |
| Glu[E] |
43 |
6.1% |
| Gly[G] |
25 |
3.5% |
| His[H] |
16 |
2.3% |
| Ile[I] |
48 |
6.8% |
| Leu[L] |
84 |
11.9% |
| Lys[K] |
58 |
8.2% |
| Met[M] |
15 |
2.1% |
| Phe[F] |
39 |
5.5% |
| Pro[P] |
21 |
3.0% |
| Ser[S] |
73 |
10.4% |
| Thr[T] |
35 |
5.0% |
| Trp[W] |
3 |
0.4% |
| Tyr[Y] |
26 |
3.7% |
| Val[V] |
37 |
5.2% |
| Total Length |
705 |
|
| Instability index (II) |
41.40 |
| This classifies the protein as unstable |
|
| Carbon |
3642 |
| Hydrogen |
5787 |
| Nitrogen |
987 |
| Oxygen |
1073 |
| Sulfur |
28 |
| Total number of atoms |
11517 |
| Formula = C3642H5787N987O1073S28 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
55990 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
55240 M-1 cm-1 |
| assuming all Cys residues are reduced |
55240 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
55990 M-1 cm-1 |
|
|
| Codon Bias |
-0.018 |
| Codon Adaptation Index |
0.117 |
| Frequency of Optimal Codons |
0.399 |
| Hydropathicity of Protein |
-0.355 |
| Aromaticity Score |
0.096 |
|
