REC114/YMR133W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 12 2.8%
Arg[R] 21 4.9%
Asn[N] 42 9.8%
Asp[D] 17 4.0%
Cys[C] 7 1.6%
Gln[Q] 27 6.3%
Glu[E] 27 6.3%
Gly[G] 13 3.0%
His[H] 4 0.9%
Ile[I] 27 6.3%
Leu[L] 35 8.2%
Lys[K] 35 8.2%
Met[M] 11 2.6%
Phe[F] 14 3.3%
Pro[P] 25 5.8%
Ser[S] 38 8.9%
Thr[T] 37 8.6%
Trp[W] 4 0.9%
Tyr[Y] 7 1.6%
Val[V] 25 5.8%
Total Length 428

Instability index
Instability index (II) 44.52
This classifies the protein as unstable

Atomic composition
Carbon 2121
Hydrogen 3437
Nitrogen 607
Oxygen 668
Sulfur 18
Total number of atoms 6851
Formula = C2121H3437N607O668S18

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 32805 M-1 cm-1
assuming NO Cys residues appear as half cystines 32430 M-1 cm-1
assuming all Cys residues are reduced 32430 M-1 cm-1
assuming all pairs of Cys residues form cystines 32805 M-1 cm-1

Aliphatic index
Aliphatic index 76.24

Coding Region Translation Calculations
Codon Bias 0.073
Codon Adaptation Index 0.128
Frequency of Optimal Codons 0.462
Hydropathicity of Protein -0.689
Aromaticity Score 0.058