Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
35 |
6.8% |
| Arg[R] |
33 |
6.4% |
| Asn[N] |
27 |
5.3% |
| Asp[D] |
27 |
5.3% |
| Cys[C] |
5 |
1.0% |
| Gln[Q] |
24 |
4.7% |
| Glu[E] |
24 |
4.7% |
| Gly[G] |
24 |
4.7% |
| His[H] |
15 |
2.9% |
| Ile[I] |
24 |
4.7% |
| Leu[L] |
47 |
9.2% |
| Lys[K] |
31 |
6.0% |
| Met[M] |
8 |
1.6% |
| Phe[F] |
20 |
3.9% |
| Pro[P] |
22 |
4.3% |
| Ser[S] |
52 |
10.1% |
| Thr[T] |
35 |
6.8% |
| Trp[W] |
7 |
1.4% |
| Tyr[Y] |
15 |
2.9% |
| Val[V] |
38 |
7.4% |
| Total Length |
513 |
|
| Instability index (II) |
42.27 |
| This classifies the protein as unstable |
|
| Carbon |
2552 |
| Hydrogen |
4043 |
| Nitrogen |
731 |
| Oxygen |
769 |
| Sulfur |
13 |
| Total number of atoms |
8108 |
| Formula = C2552H4043N731O769S13 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
61100 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
60850 M-1 cm-1 |
| assuming all Cys residues are reduced |
60850 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
61100 M-1 cm-1 |
|
|
| Codon Bias |
0.075 |
| Codon Adaptation Index |
0.148 |
| Frequency of Optimal Codons |
0.442 |
| Hydropathicity of Protein |
-0.425 |
| Aromaticity Score |
0.082 |
|
