Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
15 |
4.4% |
| Arg[R] |
20 |
5.9% |
| Asn[N] |
38 |
11.2% |
| Asp[D] |
13 |
3.8% |
| Cys[C] |
6 |
1.8% |
| Gln[Q] |
9 |
2.7% |
| Glu[E] |
14 |
4.1% |
| Gly[G] |
13 |
3.8% |
| His[H] |
17 |
5.0% |
| Ile[I] |
13 |
3.8% |
| Leu[L] |
29 |
8.6% |
| Lys[K] |
13 |
3.8% |
| Met[M] |
1 |
0.3% |
| Phe[F] |
11 |
3.3% |
| Pro[P] |
17 |
5.0% |
| Ser[S] |
73 |
21.6% |
| Thr[T] |
16 |
4.7% |
| Trp[W] |
0 |
0.0% |
| Tyr[Y] |
10 |
3.0% |
| Val[V] |
10 |
3.0% |
| Total Length |
338 |
|
| Instability index (II) |
58.16 |
| This classifies the protein as unstable |
|
| Carbon |
1572 |
| Hydrogen |
2470 |
| Nitrogen |
492 |
| Oxygen |
539 |
| Sulfur |
7 |
| Total number of atoms |
5080 |
| Formula = C1572H2470N492O539S7 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
15275 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
14900 M-1 cm-1 |
| assuming all Cys residues are reduced |
14900 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
15275 M-1 cm-1 |
|
|
| Codon Bias |
0.127 |
| Codon Adaptation Index |
0.139 |
| Frequency of Optimal Codons |
0.466 |
| Hydropathicity of Protein |
-0.839 |
| Aromaticity Score |
0.062 |
|
