Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
59 |
6.1% |
| Arg[R] |
49 |
5.0% |
| Asn[N] |
57 |
5.9% |
| Asp[D] |
55 |
5.7% |
| Cys[C] |
19 |
2.0% |
| Gln[Q] |
50 |
5.1% |
| Glu[E] |
66 |
6.8% |
| Gly[G] |
53 |
5.5% |
| His[H] |
22 |
2.3% |
| Ile[I] |
54 |
5.6% |
| Leu[L] |
99 |
10.2% |
| Lys[K] |
60 |
6.2% |
| Met[M] |
14 |
1.4% |
| Phe[F] |
39 |
4.0% |
| Pro[P] |
44 |
4.5% |
| Ser[S] |
80 |
8.2% |
| Thr[T] |
45 |
4.6% |
| Trp[W] |
13 |
1.3% |
| Tyr[Y] |
28 |
2.9% |
| Val[V] |
65 |
6.7% |
| Total Length |
971 |
|
| Instability index (II) |
34.89 |
| This classifies the protein as stable |
|
| Carbon |
4853 |
| Hydrogen |
7642 |
| Nitrogen |
1342 |
| Oxygen |
1474 |
| Sulfur |
33 |
| Total number of atoms |
15344 |
| Formula = C4853H7642N1342O1474S33 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
114345 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
113220 M-1 cm-1 |
| assuming all Cys residues are reduced |
113220 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
114345 M-1 cm-1 |
|
|
| Codon Bias |
0.180 |
| Codon Adaptation Index |
0.204 |
| Frequency of Optimal Codons |
0.510 |
| Hydropathicity of Protein |
-0.388 |
| Aromaticity Score |
0.082 |
|
