Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
50 |
5.6% |
| Arg[R] |
39 |
4.4% |
| Asn[N] |
41 |
4.6% |
| Asp[D] |
51 |
5.7% |
| Cys[C] |
7 |
0.8% |
| Gln[Q] |
37 |
4.2% |
| Glu[E] |
60 |
6.7% |
| Gly[G] |
56 |
6.3% |
| His[H] |
21 |
2.4% |
| Ile[I] |
62 |
7.0% |
| Leu[L] |
98 |
11.0% |
| Lys[K] |
42 |
4.7% |
| Met[M] |
35 |
3.9% |
| Phe[F] |
56 |
6.3% |
| Pro[P] |
26 |
2.9% |
| Ser[S] |
60 |
6.7% |
| Thr[T] |
48 |
5.4% |
| Trp[W] |
15 |
1.7% |
| Tyr[Y] |
30 |
3.4% |
| Val[V] |
56 |
6.3% |
| Total Length |
890 |
|
| Instability index (II) |
38.05 |
| This classifies the protein as stable |
|
| Carbon |
4604 |
| Hydrogen |
7112 |
| Nitrogen |
1184 |
| Oxygen |
1329 |
| Sulfur |
42 |
| Total number of atoms |
14271 |
| Formula = C4604H7112N1184O1329S42 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
127575 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
127200 M-1 cm-1 |
| assuming all Cys residues are reduced |
127200 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
127575 M-1 cm-1 |
|
|
| Codon Bias |
0.113 |
| Codon Adaptation Index |
0.158 |
| Frequency of Optimal Codons |
0.474 |
| Hydropathicity of Protein |
-0.055 |
| Aromaticity Score |
0.113 |
|
