Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
27 |
3.8% |
| Arg[R] |
28 |
4.0% |
| Asn[N] |
102 |
14.5% |
| Asp[D] |
46 |
6.5% |
| Cys[C] |
5 |
0.7% |
| Gln[Q] |
30 |
4.3% |
| Glu[E] |
23 |
3.3% |
| Gly[G] |
18 |
2.6% |
| His[H] |
21 |
3.0% |
| Ile[I] |
39 |
5.5% |
| Leu[L] |
56 |
8.0% |
| Lys[K] |
31 |
4.4% |
| Met[M] |
18 |
2.6% |
| Phe[F] |
19 |
2.7% |
| Pro[P] |
44 |
6.2% |
| Ser[S] |
110 |
15.6% |
| Thr[T] |
53 |
7.5% |
| Trp[W] |
1 |
0.1% |
| Tyr[Y] |
13 |
1.8% |
| Val[V] |
20 |
2.8% |
| Total Length |
704 |
|
| Instability index (II) |
57.86 |
| This classifies the protein as unstable |
|
| Carbon |
3290 |
| Hydrogen |
5228 |
| Nitrogen |
994 |
| Oxygen |
1151 |
| Sulfur |
23 |
| Total number of atoms |
10686 |
| Formula = C3290H5228N994O1151S23 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
25120 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
24870 M-1 cm-1 |
| assuming all Cys residues are reduced |
24870 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
25120 M-1 cm-1 |
|
|
| Codon Bias |
0.056 |
| Codon Adaptation Index |
0.133 |
| Frequency of Optimal Codons |
0.442 |
| Hydropathicity of Protein |
-0.877 |
| Aromaticity Score |
0.047 |
|
