YML122C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 11 8.7%
Arg[R] 14 11.1%
Asn[N] 7 5.6%
Asp[D] 2 1.6%
Cys[C] 8 6.3%
Gln[Q] 5 4.0%
Glu[E] 5 4.0%
Gly[G] 5 4.0%
His[H] 3 2.4%
Ile[I] 6 4.8%
Leu[L] 10 7.9%
Lys[K] 4 3.2%
Met[M] 3 2.4%
Phe[F] 6 4.8%
Pro[P] 4 3.2%
Ser[S] 15 11.9%
Thr[T] 6 4.8%
Trp[W] 2 1.6%
Tyr[Y] 6 4.8%
Val[V] 4 3.2%
Total Length 126

Instability index
Instability index (II) 62.87
This classifies the protein as unstable

Atomic composition
Carbon 629
Hydrogen 988
Nitrogen 192
Oxygen 180
Sulfur 11
Total number of atoms 2000
Formula = C629H988N192O180S11

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 20440 M-1 cm-1
assuming NO Cys residues appear as half cystines 19940 M-1 cm-1
assuming all Cys residues are reduced 19940 M-1 cm-1
assuming all pairs of Cys residues form cystines 20440 M-1 cm-1

Aliphatic index
Aliphatic index 67.46

Coding Region Translation Calculations
Codon Bias -0.027
Codon Adaptation Index 0.094
Frequency of Optimal Codons 0.364
Hydropathicity of Protein -0.356
Aromaticity Score 0.111