NDI1/YML120C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 41 8.0%
Arg[R] 22 4.3%
Asn[N] 29 5.7%
Asp[D] 20 3.9%
Cys[C] 0 0.0%
Gln[Q] 15 2.9%
Glu[E] 31 6.0%
Gly[G] 33 6.4%
His[H] 11 2.1%
Ile[I] 34 6.6%
Leu[L] 56 10.9%
Lys[K] 43 8.4%
Met[M] 6 1.2%
Phe[F] 28 5.5%
Pro[P] 26 5.1%
Ser[S] 36 7.0%
Thr[T] 31 6.0%
Trp[W] 4 0.8%
Tyr[Y] 18 3.5%
Val[V] 29 5.7%
Total Length 513

Instability index
Instability index (II) 35.71
This classifies the protein as stable

Atomic composition
Carbon 2606
Hydrogen 4112
Nitrogen 692
Oxygen 745
Sulfur 6
Total number of atoms 8161
Formula = C2606H4112N692O745S6

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 48820 M-1 cm-1

Aliphatic index
Aliphatic index 92.81

Coding Region Translation Calculations
Codon Bias 0.234
Codon Adaptation Index 0.190
Frequency of Optimal Codons 0.539
Hydropathicity of Protein -0.225
Aromaticity Score 0.097