Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
53 |
5.9% |
| Arg[R] |
56 |
6.2% |
| Asn[N] |
63 |
7.0% |
| Asp[D] |
89 |
9.9% |
| Cys[C] |
0 |
0.0% |
| Gln[Q] |
40 |
4.4% |
| Glu[E] |
113 |
12.6% |
| Gly[G] |
38 |
4.2% |
| His[H] |
11 |
1.2% |
| Ile[I] |
38 |
4.2% |
| Leu[L] |
59 |
6.6% |
| Lys[K] |
99 |
11.0% |
| Met[M] |
26 |
2.9% |
| Phe[F] |
18 |
2.0% |
| Pro[P] |
25 |
2.8% |
| Ser[S] |
74 |
8.2% |
| Thr[T] |
39 |
4.3% |
| Trp[W] |
7 |
0.8% |
| Tyr[Y] |
12 |
1.3% |
| Val[V] |
39 |
4.3% |
| Total Length |
899 |
|
| Instability index (II) |
51.26 |
| This classifies the protein as unstable |
|
| Carbon |
4361 |
| Hydrogen |
7062 |
| Nitrogen |
1298 |
| Oxygen |
1532 |
| Sulfur |
26 |
| Total number of atoms |
14279 |
| Formula = C4361H7062N1298O1532S26 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
56380 M-1 cm-1 |
|
|
| Codon Bias |
0.192 |
| Codon Adaptation Index |
0.225 |
| Frequency of Optimal Codons |
0.528 |
| Hydropathicity of Protein |
-1.279 |
| Aromaticity Score |
0.041 |
|