Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
50 |
8.6% |
| Arg[R] |
12 |
2.1% |
| Asn[N] |
27 |
4.6% |
| Asp[D] |
37 |
6.3% |
| Cys[C] |
5 |
0.9% |
| Gln[Q] |
11 |
1.9% |
| Glu[E] |
38 |
6.5% |
| Gly[G] |
56 |
9.6% |
| His[H] |
9 |
1.5% |
| Ile[I] |
35 |
6.0% |
| Leu[L] |
54 |
9.2% |
| Lys[K] |
48 |
8.2% |
| Met[M] |
10 |
1.7% |
| Phe[F] |
27 |
4.6% |
| Pro[P] |
19 |
3.3% |
| Ser[S] |
51 |
8.7% |
| Thr[T] |
35 |
6.0% |
| Trp[W] |
2 |
0.3% |
| Tyr[Y] |
11 |
1.9% |
| Val[V] |
47 |
8.0% |
| Total Length |
584 |
|
| Instability index (II) |
27.40 |
| This classifies the protein as stable |
|
| Carbon |
2763 |
| Hydrogen |
4416 |
| Nitrogen |
726 |
| Oxygen |
870 |
| Sulfur |
15 |
| Total number of atoms |
8790 |
| Formula = C2763H4416N726O870S15 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
27640 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
27390 M-1 cm-1 |
| assuming all Cys residues are reduced |
27390 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
27640 M-1 cm-1 |
|
|
| Codon Bias |
0.237 |
| Codon Adaptation Index |
0.217 |
| Frequency of Optimal Codons |
0.544 |
| Hydropathicity of Protein |
-0.073 |
| Aromaticity Score |
0.068 |
|
