YML039W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 81 4.6%
Arg[R] 71 4.0%
Asn[N] 126 7.2%
Asp[D] 103 5.9%
Cys[C] 14 0.8%
Gln[Q] 76 4.3%
Glu[E] 90 5.1%
Gly[G] 68 3.9%
His[H] 60 3.4%
Ile[I] 129 7.4%
Leu[L] 141 8.0%
Lys[K] 128 7.3%
Met[M] 31 1.8%
Phe[F] 52 3.0%
Pro[P] 112 6.4%
Ser[S] 178 10.1%
Thr[T] 135 7.7%
Trp[W] 13 0.7%
Tyr[Y] 76 4.3%
Val[V] 71 4.0%
Total Length 1755

Instability index
Instability index (II) 48.68
This classifies the protein as unstable

Atomic composition
Carbon 8780
Hydrogen 13787
Nitrogen 2431
Oxygen 2733
Sulfur 45
Total number of atoms 27776
Formula = C8780H13787N2431O2733S45

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 185615 M-1 cm-1
assuming NO Cys residues appear as half cystines 184740 M-1 cm-1
assuming all Cys residues are reduced 184740 M-1 cm-1
assuming all pairs of Cys residues form cystines 185615 M-1 cm-1

Aliphatic index
Aliphatic index 76.35

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000