Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
33 |
3.9% |
| Arg[R] |
37 |
4.4% |
| Asn[N] |
61 |
7.3% |
| Asp[D] |
50 |
6.0% |
| Cys[C] |
8 |
1.0% |
| Gln[Q] |
37 |
4.4% |
| Glu[E] |
65 |
7.8% |
| Gly[G] |
29 |
3.5% |
| His[H] |
19 |
2.3% |
| Ile[I] |
70 |
8.4% |
| Leu[L] |
89 |
10.6% |
| Lys[K] |
41 |
4.9% |
| Met[M] |
11 |
1.3% |
| Phe[F] |
44 |
5.3% |
| Pro[P] |
46 |
5.5% |
| Ser[S] |
68 |
8.1% |
| Thr[T] |
44 |
5.3% |
| Trp[W] |
10 |
1.2% |
| Tyr[Y] |
34 |
4.1% |
| Val[V] |
42 |
5.0% |
| Total Length |
838 |
|
| Instability index (II) |
39.08 |
| This classifies the protein as stable |
|
| Carbon |
4358 |
| Hydrogen |
6728 |
| Nitrogen |
1136 |
| Oxygen |
1313 |
| Sulfur |
19 |
| Total number of atoms |
13554 |
| Formula = C4358H6728N1136O1313S19 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
106160 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
105660 M-1 cm-1 |
| assuming all Cys residues are reduced |
105660 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
106160 M-1 cm-1 |
|
|
| Codon Bias |
0.046 |
| Codon Adaptation Index |
0.127 |
| Frequency of Optimal Codons |
0.443 |
| Hydropathicity of Protein |
-0.362 |
| Aromaticity Score |
0.105 |
|
