TSA1/YML028W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 19 9.7%
Arg[R] 6 3.1%
Asn[N] 8 4.1%
Asp[D] 11 5.6%
Cys[C] 2 1.0%
Gln[Q] 6 3.1%
Glu[E] 15 7.7%
Gly[G] 13 6.6%
His[H] 2 1.0%
Ile[I] 13 6.6%
Leu[L] 17 8.7%
Lys[K] 14 7.1%
Met[M] 1 0.5%
Phe[F] 11 5.6%
Pro[P] 11 5.6%
Ser[S] 8 4.1%
Thr[T] 13 6.6%
Trp[W] 3 1.5%
Tyr[Y] 5 2.6%
Val[V] 18 9.2%
Total Length 196

Instability index
Instability index (II) 29.98
This classifies the protein as stable

Atomic composition
Carbon 985
Hydrogen 1539
Nitrogen 249
Oxygen 289
Sulfur 3
Total number of atoms 3065
Formula = C985H1539N249O289S3

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 24075 M-1 cm-1
assuming NO Cys residues appear as half cystines 23950 M-1 cm-1
assuming all Cys residues are reduced 23950 M-1 cm-1
assuming all pairs of Cys residues form cystines 24075 M-1 cm-1

Aliphatic index
Aliphatic index 96.02

Coding Region Translation Calculations
Codon Bias 0.780
Codon Adaptation Index 0.714
Frequency of Optimal Codons 0.870
Hydropathicity of Protein -0.025
Aromaticity Score 0.097