Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
78 |
7.3% |
| Arg[R] |
60 |
5.6% |
| Asn[N] |
58 |
5.5% |
| Asp[D] |
92 |
8.7% |
| Cys[C] |
3 |
0.3% |
| Gln[Q] |
48 |
4.5% |
| Glu[E] |
76 |
7.1% |
| Gly[G] |
109 |
10.3% |
| His[H] |
15 |
1.4% |
| Ile[I] |
55 |
5.2% |
| Leu[L] |
54 |
5.1% |
| Lys[K] |
73 |
6.9% |
| Met[M] |
16 |
1.5% |
| Phe[F] |
23 |
2.2% |
| Pro[P] |
39 |
3.7% |
| Ser[S] |
91 |
8.6% |
| Thr[T] |
74 |
7.0% |
| Trp[W] |
15 |
1.4% |
| Tyr[Y] |
23 |
2.2% |
| Val[V] |
61 |
5.7% |
| Total Length |
1063 |
|
| Instability index (II) |
36.40 |
| This classifies the protein as stable |
|
| Carbon |
4951 |
| Hydrogen |
7861 |
| Nitrogen |
1467 |
| Oxygen |
1694 |
| Sulfur |
19 |
| Total number of atoms |
15992 |
| Formula = C4951H7861N1467O1694S19 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
116895 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
116770 M-1 cm-1 |
| assuming all Cys residues are reduced |
116770 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
116895 M-1 cm-1 |
|
|
| Codon Bias |
0.181 |
| Codon Adaptation Index |
0.199 |
| Frequency of Optimal Codons |
0.511 |
| Hydropathicity of Protein |
-0.832 |
| Aromaticity Score |
0.057 |
|
