Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
29 |
4.5% |
| Arg[R] |
23 |
3.5% |
| Asn[N] |
77 |
11.8% |
| Asp[D] |
60 |
9.2% |
| Cys[C] |
6 |
0.9% |
| Gln[Q] |
32 |
4.9% |
| Glu[E] |
43 |
6.6% |
| Gly[G] |
26 |
4.0% |
| His[H] |
9 |
1.4% |
| Ile[I] |
25 |
3.8% |
| Leu[L] |
42 |
6.5% |
| Lys[K] |
49 |
7.5% |
| Met[M] |
13 |
2.0% |
| Phe[F] |
28 |
4.3% |
| Pro[P] |
31 |
4.8% |
| Ser[S] |
82 |
12.6% |
| Thr[T] |
35 |
5.4% |
| Trp[W] |
3 |
0.5% |
| Tyr[Y] |
9 |
1.4% |
| Val[V] |
28 |
4.3% |
| Total Length |
650 |
|
| Instability index (II) |
47.58 |
| This classifies the protein as unstable |
|
| Carbon |
3080 |
| Hydrogen |
4842 |
| Nitrogen |
898 |
| Oxygen |
1092 |
| Sulfur |
19 |
| Total number of atoms |
9931 |
| Formula = C3080H4842N898O1092S19 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
30285 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
29910 M-1 cm-1 |
| assuming all Cys residues are reduced |
29910 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
30285 M-1 cm-1 |
|
|
| Codon Bias |
-0.006 |
| Codon Adaptation Index |
0.146 |
| Frequency of Optimal Codons |
0.413 |
| Hydropathicity of Protein |
-1.031 |
| Aromaticity Score |
0.062 |
|
