Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
23 |
5.8% |
| Arg[R] |
10 |
2.5% |
| Asn[N] |
16 |
4.1% |
| Asp[D] |
8 |
2.0% |
| Cys[C] |
4 |
1.0% |
| Gln[Q] |
12 |
3.0% |
| Glu[E] |
7 |
1.8% |
| Gly[G] |
18 |
4.6% |
| His[H] |
6 |
1.5% |
| Ile[I] |
44 |
11.2% |
| Leu[L] |
66 |
16.8% |
| Lys[K] |
12 |
3.0% |
| Met[M] |
5 |
1.3% |
| Phe[F] |
37 |
9.4% |
| Pro[P] |
19 |
4.8% |
| Ser[S] |
39 |
9.9% |
| Thr[T] |
15 |
3.8% |
| Trp[W] |
8 |
2.0% |
| Tyr[Y] |
20 |
5.1% |
| Val[V] |
25 |
6.3% |
| Total Length |
394 |
|
| Instability index (II) |
39.61 |
| This classifies the protein as stable |
|
| Carbon |
2159 |
| Hydrogen |
3284 |
| Nitrogen |
484 |
| Oxygen |
527 |
| Sulfur |
9 |
| Total number of atoms |
6463 |
| Formula = C2159H3284N484O527S9 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
74050 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
73800 M-1 cm-1 |
| assuming all Cys residues are reduced |
73800 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
74050 M-1 cm-1 |
|
|
| Codon Bias |
0.096 |
| Codon Adaptation Index |
0.115 |
| Frequency of Optimal Codons |
0.454 |
| Hydropathicity of Protein |
0.874 |
| Aromaticity Score |
0.165 |
|
