Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
12 |
3.0% |
| Arg[R] |
13 |
3.3% |
| Asn[N] |
25 |
6.3% |
| Asp[D] |
20 |
5.1% |
| Cys[C] |
5 |
1.3% |
| Gln[Q] |
14 |
3.5% |
| Glu[E] |
24 |
6.1% |
| Gly[G] |
6 |
1.5% |
| His[H] |
9 |
2.3% |
| Ile[I] |
29 |
7.3% |
| Leu[L] |
40 |
10.1% |
| Lys[K] |
43 |
10.9% |
| Met[M] |
12 |
3.0% |
| Phe[F] |
23 |
5.8% |
| Pro[P] |
13 |
3.3% |
| Ser[S] |
45 |
11.4% |
| Thr[T] |
18 |
4.6% |
| Trp[W] |
0 |
0.0% |
| Tyr[Y] |
15 |
3.8% |
| Val[V] |
29 |
7.3% |
| Total Length |
395 |
|
| Instability index (II) |
37.73 |
| This classifies the protein as stable |
|
| Carbon |
2056 |
| Hydrogen |
3282 |
| Nitrogen |
534 |
| Oxygen |
601 |
| Sulfur |
17 |
| Total number of atoms |
6490 |
| Formula = C2056H3282N534O601S17 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
22600 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
22350 M-1 cm-1 |
| assuming all Cys residues are reduced |
22350 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
22600 M-1 cm-1 |
|
|
| Codon Bias |
0.007 |
| Codon Adaptation Index |
0.136 |
| Frequency of Optimal Codons |
0.431 |
| Hydropathicity of Protein |
-0.282 |
| Aromaticity Score |
0.096 |
|
