Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
51 |
5.8% |
| Arg[R] |
44 |
5.0% |
| Asn[N] |
60 |
6.8% |
| Asp[D] |
41 |
4.6% |
| Cys[C] |
14 |
1.6% |
| Gln[Q] |
33 |
3.7% |
| Glu[E] |
70 |
7.9% |
| Gly[G] |
31 |
3.5% |
| His[H] |
15 |
1.7% |
| Ile[I] |
53 |
6.0% |
| Leu[L] |
78 |
8.8% |
| Lys[K] |
54 |
6.1% |
| Met[M] |
23 |
2.6% |
| Phe[F] |
34 |
3.8% |
| Pro[P] |
39 |
4.4% |
| Ser[S] |
95 |
10.7% |
| Thr[T] |
60 |
6.8% |
| Trp[W] |
11 |
1.2% |
| Tyr[Y] |
26 |
2.9% |
| Val[V] |
54 |
6.1% |
| Total Length |
886 |
|
| Instability index (II) |
48.72 |
| This classifies the protein as unstable |
|
| Carbon |
4406 |
| Hydrogen |
6972 |
| Nitrogen |
1206 |
| Oxygen |
1383 |
| Sulfur |
37 |
| Total number of atoms |
14004 |
| Formula = C4406H6972N1206O1383S37 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
100115 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
99240 M-1 cm-1 |
| assuming all Cys residues are reduced |
99240 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
100115 M-1 cm-1 |
|
|
| Codon Bias |
0.004 |
| Codon Adaptation Index |
0.118 |
| Frequency of Optimal Codons |
0.411 |
| Hydropathicity of Protein |
-0.429 |
| Aromaticity Score |
0.080 |
|
